Install pipeline

1) Installing NGS_DNA

We first have to load EasyBuild, this can be done with this command
module load EasyBuild
The NGS_DNA pipeline has a lot of dependencies, these are handled by easybuild when the --robot command is executed (all the dependencies can be found here). Since we have also our own repo we have to give the path to that also. There can be multiple paths to easybuild configs, just separate them by colon.
Note: the order in which you give the paths are important! To original easybuild path can be left empty (just a colon is enough)
eb NGS_DNA-3.2.3.eb --robot --robot-paths=${pathToMYeasybuild}/easybuild-easyconfigs/easybuild/easyconfigs/:
Note: some software cannot be downloaded automagically due to for example licensing or technical issues and the build will fail initially. In these cases you will have to download manually and copy the sources to ${HPC_ENV_PREFIX}/sources/[a-z]/NameOfTheSoftwarePackage/ This is the case for example for Java. Therefore:
scp jdk-7u80-linux-x64.tar.gz [email protected]:${root}/apps/sources/j/Java/
scp jdk-8u45-linux-x64.tar.gz [email protected]:${root}/apps/sources/j/Java/
but also tools as GATK, Tabix and snpEff should be download manually:
scp GATK-3.5.tar.gz [email protected]: ${root}/apps/sources/g/GATK/
scp tabix.0.18.6.tar.gz [email protected]: ${root}/apps/sources/t/tabix
scp snpEff-4.1g.tar.gz [email protected]: ${root}/apps/sources/s/snpEff/
change config file of snpEff: data.dir=./data should be data.dir=${root}/apps/data/snpEff

2) Installing the necessary resources (reference genome, dbSNP etc)

Logout and login again. Run the script NGS_resources to install the required resources, you can download the scripts here
This script will download parts of the 2.8 bundle from the GATK server
Note: Sometimes the GATK ftp server can be down/instable, try it a couple of times

3) Creating workdir structure